Genus Sorbus, with all its representatives, is morphologically divergent, and it is possible on the basis of phenotypic characteristics to determine the relative taxonomic background of individuals within a population in a particular locality. Representatives of the genus are characteristic of forest biodiversity of Serbia, although they can be found as solitary individuals in certain habitats of various degrees of forestation. The service tree (Sorbus domestica), The wild service tree (S. torminalis), The whitebeam (S. aria) are the names of the representatives of the dominant species of genus Sorbus which can be find in the territory of southern, southeastern and central Serbia, and which are used in human nutrition, as well as a unique species wood for making different instruments, materials, fuel, furniture, tools, etc. In our study we analyzed the intra-loci polymorphism in the genus Sorbus specimens sampled from 7 localities of the Republic of Serbia- Bukovik I and II, Various locations (the territory of which is determined by the point-Bor, Čačak, Novi Pazar, Raška), Orovica, Kopaonik mountain, Roška and Spa Žirave. This type of analysis was necessary to determine the initial stage intrapopulation diversity at the level of the analyzed loci (MSS1, MSS6, MSS9, MSS13 and MSS16), which are characteristic polymorphic markers. Our results showed that there is a high degree of allelic polymorphism within certain loci (MSS16) and that there are significant differences between the populations themselves (Bukovik and second lowest value of allelic polymorphism P_asr=26; samples taken from various locations (Bor, Čačak, Novi Pazar, Raška) are of the largest value of allelic polymorphism P_asr=66.8). The results represent a platform for further development of molecular genetic analysis of the genus in Serbia, in order to preserve the gene pool and for breeding purposes.