The leaf cuticular waxes of 3 poplar clones (Populus euramericana cl. Pannonia (M1), and Populus deltoides cl. PE 19/66 and B229 (Bora)) were characterized by gas chromatography–mass spectrometry method. Poplar clones grown under identical environmental conditions showed almost identical chemical content of organic compounds within analyzed leaf cuticular wax. The dominant compound was nonacosane, with range from 72.61% ± 0.02 quantified in Pannonia clone to 78.40% ± 0.35 in B229 clone, in total cuticular wax content. Other identified compounds were hexacosane, untriacontane, octacosane, tetradecanal and triacontane; the last, triacontane, was present in very small percentage in wax content, around 1% in all three clones.