Differences between genotypes are considered to be the most important requisite for a resilient urban forest. Analyses of physiological traits, such as leaf water potential and leaf gas exchange could provide useful insight into the capacity of different species and genotypes to grow in harsh urban environments. In the present study, a variation of midday (Ψ_md) and predawn (Ψ_pd) leaf water potential, net photosynthesis (A), rate of transpiration (E), stomatal conductance (g_s), and intercellular CO₂ concentration (C_i) of seven Silver linden genotypes (Tilia tomentosa Moench), planted in the urban environment in Novi Sad, were examined. Analysis of variance and LSD tests were used to show differences between studied silver linden genotypes. The results showed significant differences for all observed leaf gas exchange parameters (A, E, g_s, C_i, Ψ_pd and Ψ_md) between genotypes. The results indicate better physiological performances of genotypes T3, in comparison to other observed genotypes under the prevailing environmental condition of the studied site in the urban environment.