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Poplar
2006, Issue 177-178, p. 88-105

Original scientific paper
UDC: 631.528: 546.48: 582.681.81

The Influence of Cadmium on the In Vitro Growth of Poplar Shoots from the Leuce Section


Marina Katanić 1*, Zoran Tomović 2, Andrej Pilipović 1, Saša Orlović 1, Borivoje Krstić 3


1 University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
2 Public Enterprise "Vojvodinašume", Petrovaradin, Serbia
3 University of Novi Sad, Faculty of Sciences, Novi Sad, Serbia

Corresponding author:
Marina Katanić, E-mail: katanicm@uns.ac.rs


Abstract

The influence of cadmium presence on growth of shoots of four poplar clones from section Leuce cultivated in tissue culture is presented in this work. Shoots of clones L-12, L-80, L-111/81 and LBM were multiplicated by micropropagation on ACM (Aspen Culture Medium) with 0.5 mg/l 6-benzylaminopurine (BAP) and 0.02 mg/l a-naphthaleneacetic acid (NAA). Terminal shoots, about 1.5 cm long, were cultivated 30 days on the ACM medium with addition of 0.1 mM and 0.5 mM of Cd. Parameters examinated in shoot cultures included: length of the main shoot, shoot multiplication (number of axillary shoots per explant) and fresh mass. It was observed that the addition of 0.5 mM Cd (here considered as high concentration) was toxic for shoots of all investigated clones. The symptoms included were decrease of growth vigor and leaf clorosis. Length, fresh mass and shoot multiplication were decreased compared to control. Low concentration of cadmium (0.1 mM) had stimulatory effects on fresh mass (in total) of investigated clones, especially for clone L-111/81. These results indicate possible use of investigated clones in phytoremediation of soils that contain lower concentration of cadmium, especially clone L-111/81 which was assumed as the most suitable for this purpose. 


Keywords: phytoremediation, poplars, clon, growth, cadmium, in vitro
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University of Novi Sad
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